Li lab published a preprint in bioRxiv entitled “Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis.” In this research article, an expandable, 3D branching ureteric bud (UB) organoid culture model has been generated. The UB organoid can be derived from primary UB progenitors from mouse and human fetal kidneys, or generated de novo from human pluripotent stem cells. UB organoids differentiate into collecting duct (CD) organoids in vitro, with differentiated cell types adopting spatial assemblies reflective of the adult kidney collecting system. Aggregating 3D-cultured nephron progenitor cells with UB organoids in vitro results in a reiterative process of branching morphogenesis and nephron induction, similar to kidney development. Combining efficient gene editing with the UB organoid model will facilitate an enhanced understanding of development, regeneration and diseases of the mammalian collecting system.

Link to the preprint: https://www.biorxiv.org/content/10.1101/2020.04.27.049031v1